PLAQUE ASSAY PROCEDURE FOR COLORADO TICK FEVER VIRUS

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Plaque Assay Procedure for Colorado Tick Fever Virus.

Deig, E. Frank (University of California, Berkeley), and H. M. S. Watkins. Plaque assay procedure for Colorado tick fever virus. J. Bacteriol. 88:42-47. 1964.-A reproducible plaque assay procedure is described for the quantitation of Colorado tick fever virus in a cell line established from embryonic hamster tissue. Under the best conditions, plaques approximately 4 mm in diameter were formed a...

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Colorado Tick Fever and Dengue

Six human beings were inoculated with dengue and developed typical disease. Two of these were reinoculated and proved immune. The remaining four were later inoculated with Colorado tick fever. Three developed typical disease. The fourth, who remained well, has previously lived in an endemic area (Colorado). One patient was inoculated with Colorado tick fever first and later with dengue. He deve...

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The Experimental Transmission of Colorado Tick Fever

1. The symptoms, history of tick bite, characteristic fever curve, and white blood cell picture should enable the physician to make a diagnosis of Colorado tick fever in nearly every case. 2. The typical white blood cell picture is a depression of the total leucocytes with a shift to the left of the granulocytes. Basophilic cytoplasmic bodies appear occasionally in lymphocytes 3 to 4 days after...

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The Etiology of Colorado Tick Fever

1. The infectious agent of Colorado tick fever filtered through 181 mmicro membranes caused infection in hamsters and one volunteer. 2. In five out of seven instances, hamsters were infected initially with serum filtered through 24 mmicro membranes or the tick fever appeared on serial transfer. 3. We could not infect two volunteers with 24 mmicro filtrates, though these filtrates did cause infe...

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Diagnosis of Colorado tick fever virus infection by enzyme immunoassays for immunoglobulin M and G antibodies.

An immunoglobulin M (IgM) capture enzyme immunoassay technique was adapted for the detection of antibody to Colorado tick fever virus in sera from 84 individuals for whom diagnosis had been confirmed by virus isolation or neutralization test. Titers were compared with those for IgG and neutralizing antibodies in these Colorado tick fever cases. IgM antibody titers were higher than neutralizing ...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1964

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.88.1.42-47.1964